c1 plate based scrna seq read count data Search Results


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Experimental design included dissociation of E14.5 mouse kidney cells, <t>scRNA-Seq</t> data generation with Chromium <t>10X</t> Genomics, Drop-Seq and Fluidigm HT 800 cell IFC platforms, unsupervised bioinformatics classification of the resulting scRNA-Seq profiles, supervised harmonization of the three datasets and downstream cell-population level analyses.
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Experimental design included dissociation of E14.5 mouse kidney cells, <t>scRNA-Seq</t> data generation with Chromium <t>10X</t> Genomics, Drop-Seq and Fluidigm HT 800 cell IFC platforms, unsupervised bioinformatics classification of the resulting scRNA-Seq profiles, supervised harmonization of the three datasets and downstream cell-population level analyses.
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10X Genomics distinct scrna seq platforms
Experimental design included dissociation of E14.5 mouse kidney cells, <t>scRNA-Seq</t> data generation with Chromium <t>10X</t> Genomics, Drop-Seq and Fluidigm HT 800 cell IFC platforms, unsupervised bioinformatics classification of the resulting scRNA-Seq profiles, supervised harmonization of the three datasets and downstream cell-population level analyses.
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Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of SCRN2 gene silencing results, individual duplex components or plasmids are also available upon request.
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Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of SCRN1 gene silencing results, individual duplex components or plasmids are also available upon request.
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N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of SCRN2 gene silencing results, individual duplex components or plasmids are also available upon request.
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N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of SCRN1 gene silencing results, individual duplex components or plasmids are also available upon request.
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N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of SCRN3 gene silencing results, individual duplex components or plasmids are also available upon request.
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Experimental design included dissociation of E14.5 mouse kidney cells, scRNA-Seq data generation with Chromium 10X Genomics, Drop-Seq and Fluidigm HT 800 cell IFC platforms, unsupervised bioinformatics classification of the resulting scRNA-Seq profiles, supervised harmonization of the three datasets and downstream cell-population level analyses.

Journal: Developmental biology

Article Title: Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf

doi: 10.1016/j.ydbio.2017.11.006

Figure Lengend Snippet: Experimental design included dissociation of E14.5 mouse kidney cells, scRNA-Seq data generation with Chromium 10X Genomics, Drop-Seq and Fluidigm HT 800 cell IFC platforms, unsupervised bioinformatics classification of the resulting scRNA-Seq profiles, supervised harmonization of the three datasets and downstream cell-population level analyses.

Article Snippet: In this report we use three independent platforms, Drop-Seq, Chromium 10x Genomics and Fluidigm C1, to carry out single cell RNA-Seq (scRNA-Seq) analysis of the E14.5 mouse kidney.

Techniques:

A) De novo identified cell populations from the software ICGS are shown for each scRNA-Seq platform. The displayed heatmaps were produced by the MarkerFinder algorithm, downstream of the ICGS population predictions, with yellow indicating high relative gene expression and blue or black, low or no gene expression in the associated genes (rows). Prior established embryonic kidney marker genes corresponding to compartments are shown in panel C. Text to the left of each heatmap indicates the statistical enrichment of genes from the Drop-Seq ICGS analysis for the 16 identified populations (MarkerFinder) using the embedded gene-set enrichment analysis tool GO-Elite in AltAnalyze. B–C) t-SNE plot derived from the ICGS heatmaps in panel A, where each dot represents individual cells colored according to its B) ICGS cluster annotation or C) prior established population specific genes. CD: Collecting duct, UT: Ureteric Tip, LOH: Loop of Henle, RV: Renal vesicle, DCSB: Distal comma shaped body, Pod: podocyte, PT: Proximal Tubule, PA: Pre-tubular aggregate, CM: Cap mesenchyme, Endo: Endothelium, NZS: Nephrogenic Stroma, CS: Cortical Stroma.

Journal: Developmental biology

Article Title: Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf

doi: 10.1016/j.ydbio.2017.11.006

Figure Lengend Snippet: A) De novo identified cell populations from the software ICGS are shown for each scRNA-Seq platform. The displayed heatmaps were produced by the MarkerFinder algorithm, downstream of the ICGS population predictions, with yellow indicating high relative gene expression and blue or black, low or no gene expression in the associated genes (rows). Prior established embryonic kidney marker genes corresponding to compartments are shown in panel C. Text to the left of each heatmap indicates the statistical enrichment of genes from the Drop-Seq ICGS analysis for the 16 identified populations (MarkerFinder) using the embedded gene-set enrichment analysis tool GO-Elite in AltAnalyze. B–C) t-SNE plot derived from the ICGS heatmaps in panel A, where each dot represents individual cells colored according to its B) ICGS cluster annotation or C) prior established population specific genes. CD: Collecting duct, UT: Ureteric Tip, LOH: Loop of Henle, RV: Renal vesicle, DCSB: Distal comma shaped body, Pod: podocyte, PT: Proximal Tubule, PA: Pre-tubular aggregate, CM: Cap mesenchyme, Endo: Endothelium, NZS: Nephrogenic Stroma, CS: Cortical Stroma.

Article Snippet: In this report we use three independent platforms, Drop-Seq, Chromium 10x Genomics and Fluidigm C1, to carry out single cell RNA-Seq (scRNA-Seq) analysis of the E14.5 mouse kidney.

Techniques: Software, Produced, Expressing, Marker, Derivative Assay

A) ICGS delineated cellular heterogeneity in the top ~2,000 DropSeq captured barcodes, segregated into 16 populations. B–C) Supervised classification of Fluidigm-800 chip captured libraries and 10X Genomics Chromium In-Drop barcodes using B) K-nearest neighbor (knn) classification against the Drop-Seq population centroids or C) MarkerFinder classified cells (rather than genes) for the top 862 population-specific genes from the Drop-Seq analysis. The upper panel displays the 862 Drop-Seq population specific genes and lower panel the top de novo MarkerFinder-gene results obtained following cell-population assignment. D) Comparison of population-specific genes jointly identified by all three scRNA-Seq technological platforms from the knn or MarkerFinder analysis. E) Number of genes jointly identified population-specific genes for each individual population for the two classification approaches. DCSB: Distal comma shaped body.

Journal: Developmental biology

Article Title: Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf

doi: 10.1016/j.ydbio.2017.11.006

Figure Lengend Snippet: A) ICGS delineated cellular heterogeneity in the top ~2,000 DropSeq captured barcodes, segregated into 16 populations. B–C) Supervised classification of Fluidigm-800 chip captured libraries and 10X Genomics Chromium In-Drop barcodes using B) K-nearest neighbor (knn) classification against the Drop-Seq population centroids or C) MarkerFinder classified cells (rather than genes) for the top 862 population-specific genes from the Drop-Seq analysis. The upper panel displays the 862 Drop-Seq population specific genes and lower panel the top de novo MarkerFinder-gene results obtained following cell-population assignment. D) Comparison of population-specific genes jointly identified by all three scRNA-Seq technological platforms from the knn or MarkerFinder analysis. E) Number of genes jointly identified population-specific genes for each individual population for the two classification approaches. DCSB: Distal comma shaped body.

Article Snippet: In this report we use three independent platforms, Drop-Seq, Chromium 10x Genomics and Fluidigm C1, to carry out single cell RNA-Seq (scRNA-Seq) analysis of the E14.5 mouse kidney.

Techniques:

A) Cell-population annotation predictions assigned from literature and GUDMAP gene-set annotations. Corresponding kidney compartments are colored according to the ICGS cell cluster colors. B) t-SNE analysis of the harmonized knn classified cell states using the de novo identified MarkerFinder genes for each scRNA-Seq platform. The number of cells present in the plot are indicated. C) Gene expression bar chart (log2) for prior annotated kidney developmental marker genes from the knn classified datasets. D) Comparison of population-specific genes consistently identified by two or more scRNA-Seq platforms or that are specific to a single platform by MarkerFinder-gene analysis.

Journal: Developmental biology

Article Title: Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf

doi: 10.1016/j.ydbio.2017.11.006

Figure Lengend Snippet: A) Cell-population annotation predictions assigned from literature and GUDMAP gene-set annotations. Corresponding kidney compartments are colored according to the ICGS cell cluster colors. B) t-SNE analysis of the harmonized knn classified cell states using the de novo identified MarkerFinder genes for each scRNA-Seq platform. The number of cells present in the plot are indicated. C) Gene expression bar chart (log2) for prior annotated kidney developmental marker genes from the knn classified datasets. D) Comparison of population-specific genes consistently identified by two or more scRNA-Seq platforms or that are specific to a single platform by MarkerFinder-gene analysis.

Article Snippet: In this report we use three independent platforms, Drop-Seq, Chromium 10x Genomics and Fluidigm C1, to carry out single cell RNA-Seq (scRNA-Seq) analysis of the E14.5 mouse kidney.

Techniques: Expressing, Marker

Heatmaps using data from all three scRNA-seq platforms show Gdnf expression by stromal cells. Six2, Cited1 and Crym are markers of cap mesenchyme (CM), while Meis1, Foxd1, Crabp1 and Aldh1a2 are expressed in stroma. As expected, cells that clustered with cap mesenchyme as determined by these markers as well as complete gene expression signatures often showed expression of Gdnf. Surprisingly, many cells that strongly clustered with the stromal cell compartment also showed robust Gdnf expression.

Journal: Developmental biology

Article Title: Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf

doi: 10.1016/j.ydbio.2017.11.006

Figure Lengend Snippet: Heatmaps using data from all three scRNA-seq platforms show Gdnf expression by stromal cells. Six2, Cited1 and Crym are markers of cap mesenchyme (CM), while Meis1, Foxd1, Crabp1 and Aldh1a2 are expressed in stroma. As expected, cells that clustered with cap mesenchyme as determined by these markers as well as complete gene expression signatures often showed expression of Gdnf. Surprisingly, many cells that strongly clustered with the stromal cell compartment also showed robust Gdnf expression.

Article Snippet: In this report we use three independent platforms, Drop-Seq, Chromium 10x Genomics and Fluidigm C1, to carry out single cell RNA-Seq (scRNA-Seq) analysis of the E14.5 mouse kidney.

Techniques: Expressing